Serial Cloner can be downloaded as freeware for Mac and Windows OS and is definitely worth having if you carry out PCR and cloning on a regular basis. Of course, if you are amplifying your target from a plasmid, the risk of unspecific PCR products will be lower. National Centre for Biotechnology Information (NCBI)ĭid you know that the NCBI website can be used for so much more than literature and BLAST searches? At primer blast you can search for suitable primer pairs (much like Primer3), or you can enter specific primer sequences and check their specificity by performing a primer blast, helping you to avoid off-target amplification. BLASTing your primers against your genome of interest might be particularly useful if you are trying to amplify a gene that exists as part of a multigene family if your template is total genomic DNA. You can also use Primer3 to design hybridization probes for use in PCR-ELISA and other probe-based applications.Ģ. You can choose to refine your results in several ways, including the size of your desired PCR product, primer size, Tm range and other parameters. You simply paste in your target sequence and search for primers. Primer3 is a very simple yet efficient online interface for primer design. Looking for new PCR reagents and equipment.
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